Ion Channel Types - Stem cells

Stem Cells

Immunofluorescent staining for F-actin filaments (green) and nuclei (blue) in neonatal cardiomyocytes (Source: Wikimedia)

Qube and QPatch can run succesfull experiments on stem cells, however be aware that the ion channel expression changes with the maturity of the iPSC cells and iPSC cells often contains a mix of cells. We expect that to be improved going forward.

 

We recommend using QPatch for stem cell work since you can obtain good gigaseals and high success rates in physiological solutions, wherease you need flouride on Qube. Alternative platform on the market use seal enhancing agents to obtain decent seals. QPatch can run experiments on iPSC stem cells and primary cells with a gigaseal rate above 95% and a success rate up to 70% (Franz et al. 2017).

 

Optimal cell culture procedures are key to obtain high quality experiments and the success rate is dependent of the cell density. The lower limit for the cell density varies between cells provided from different vendors and even from batch to batch.

 

Below is liste some of the publications for iPSC Cardiomyocytes and Neurons

 


iPSC Cardiomyocytes

Posters

  • View Voltage- and current clamp on induced pluripotent cardiomyocytes with automated patch clamp
    Year: 2017 First author: Lindquist, A
  • View Electrophysiological properties of iCell cardiomyocytes obtained by automated patch clamp
    Year: 2011 First author: Schrøder et al., 2011
  • View Exploring stem cell-derived cardiomyocytes with automated patch clamp techniques
    Year: 2012 First author: Schrøder et al., 2012

Reports

  • View Cor.4U on QPatch
    Year: 2013 First author: Friis et al., 2013
  • View Cor.At cells embryonic stem cell derived cardiomyocytes on QPatch
    Year: 2009 First author: Schrøder et al., 2009

iPSC Neurons

Posters

  • View Electrophysiological characterization of human induced pluripotent stem cell-derived dopaminergic neurons using manual and automated patch clamp systems
    Year: 2016 First author: Franz, D

Papers

  • View Automated and manual patch clamp data of human induced pluripotent stem cell-derived dopaminergic neurons
    Year: 2017 First author: Franz, D
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