Stable recordings of P2X2/3 pharmacology on Qube 384


Application report


Kim Boddum & Anne Henriksen



The ATP-gated P2X2 and P2X3 receptors are cation-permeable
ligand-gated ion channels that open in response to extracellular adenosine 5′-triphosphate (ATP) binding. They are widely expressed in the body, including the peripheral nervous system, making them an interesting target against pain1–3.

When P2X2 and P2X3 receptors are expressed together, the functional receptors will be trimeric3 and function either as P2X2/3 heteromers, P2X2 homomers, or P2X3 homomers. The ratio between P2X2 and P2X3 homomers and P2X2/3 heteromers vary from cell to cell, so to limit the variation between sites, only multihole QChips with ten patch holes per site are used in the assays.

P2X2 and P2X3 receptors display fast and extensive desensitization, making assays vulnerable to rundown due to poor compound washout or insufficient recovery. Here we show an assay with high stability, high success rates, and low rundown.

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