Optical modulation of ion channels




Kim Boddum, Peder Skafte-Pedersen, Jens Henneke, Daniel Sauter, Jiaye Zhang, Sandra Wilson



Optical modulation of ion channels is traditionally studied using a manual patch clamp system combined with a light source. This approach, however, is limited by a very low throughput. In the present work, we show data recorded using a 384-well based automated patch clamp system equipped with 384 integrated light sources (Qube Opto 384).

In this study we evaluated Channelrhodopsin 2 (ChR2), a light-sensitive non-selective cation channel permeable to Na+, K+ and Ca2+ opened upon illumination (Berndt et al., 2012). Furthermore, we employed the chloride-conducting channelrhodopsin (iC++, Govorunova et al., 2015), which was developed from a non-selective cation conducting channelrhodopsin through a mutational approach.

Compound activation by light enables the pharmacological manipulation of receptors, ion channels and other proteins with a high degree of temporal control. We used caged GABA (Rubi-GABA, Zayat et al., 2003) to study the light activation of ligands, in combination with the microfluidic flow channel of the QChip 384, to give a higher degree of experimental control.

Go to journal

Get in Touch

We strive to provide the best for our customers, and we are always ready to help. Please let us know if you have a question for us.

Follow us