Measuring TRPV3 channel using the QPatch

HEK-TRPV3 is stable on QPatch and experiments yielded high success rates, high-quality gigaseals and good recordings of the ion channel current, although TRPV3 can be a challenging channel and more experiments need to be done to fully understand its polymodal nature. The temperature control feature provided more control over the channel as at 34oC data produced were more consistent and with less variation of phenotypes. The half maximal effective and inhibitory concentrations (EC50 and IC50) found in this study are comparable to previously reported values.

Advantages of the robust, temperature-optimized TRPV3 assay on QPatch:

• High success rates & data quality: Stable HEK-TRPV3 expression yielded consistent gigaseal formation and high-quality currents
• Temperature control improves consistency: Running experiments at 34 °C reduced phenotype variability and enhanced both activation and block potency
• Reproducible EC₅₀/IC₅₀ values: Observed half-maximal concentrations align closely with literature, validating the assay’s accuracy
• Higher throughput & precision: Automated patch clamp allows more rapid screening compared to manual methods without sacrificing reliability