Large Molecules: Wnt signal activation
Journal
Application Report
Author(s)
Year
2022
It was possible to obtain both manual and automated patch clamp (APC) recordings of the labile and scarce Wnt proteins (350-400 AA, 35-45 kDa) after thorough optimisation of compound handling.
Here we highlight aspects of handling of Wnt proteins optimised for APC testing, which could also be applied to other large molecules (e.g. toxins, peptides, antibodies).
Keywords: Large molecules