hiPSC-derived cardiomyocytes – Nav1.5 compound screening on QPatch® II

The Na_V1.5 channel is critical in maintaining the physiological electrical function in cardiomyocytes, as it is the heart’s main depolarising current. Here we focus on the Na_V1.5 channel in human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as an important target for cardiac-drug and safety screening.

• The hiPSC-CMs Na_V1.5 compound screening was performed with assay success rate (quality filters) up to 60% and ~ 20 useful experiments per 48 recording site plate.

• The study demonstrates that it is possible to screen compounds on hiPSC-CMs using the QPatch II.

• The experimental success rate of hiPSC-CMs assays was variable depending on the quality and maturation of the cell population.