High throughput screening for mode-of-action on Nav1.7
Journal
Application Report
Author(s)
Year
2020
- Constantly high success rates (99% in 3 experiments)
- Giga-ohm seal resistances in almost all wells
- Very low data spread
- All CV values of DMSO control below 10%
- No false positives with cutoff criteria 3 X SD
- Zā constantly greater 0.75
- Discrimination between different modes of inhibition (state-dependency)
- Up to 384 individual IC50 values (3072 data points) determined in just one experiment
- Using Qube in combination with Stacker it would be possible to determine up to 7680 IC50 (60,000 data points) per 24 hours