High throughput assay for Kv1.5 on Qube 384
KV1.5 channels conduct the ultra-rapid delayed rectifier current (IKur) that contributes to action potential repolarization of the heart. Mutations in the encoding gene (KCNA5) have been related to both atrial fibrillation and sudden cardiac death. KV1.5 is also found in other tissues, such as vascular smooth muscle, CNS, microglia, and Schwann cells (Clarkson et Al., 1998).
The pharmacological blockade of KV1.5 has been suggested as a prevention and treatment of various arrhythmia (Karczewski et al., 2009) In the present study, we present an assay suitable for a high throughput screening campaign.