Breaking barriers in lysosomal electrophysiology

Automated Patch Clamp (APC) of lysosomal ion channels was achieved using enlarged lysosomes (LYSO-Prep™, Oria Bioscience), enabling measurement of ion channel activity such as TRPML1. Lysosomes were successfully captured on two Sophion systems, with success rates of 69.9 ± 3.2% on the QPatch^® 48 and 74.6 ± 2.6% on the Qube® 384. The lysosomal recordings showed stable seals and robust TRPML1 current responses. Agonist concentration–response curves produced EC50 values that were reproducible on both platforms and in good agreement with published data8. Based on these results, Sophion’s medium-and high-throughput APC systems provide a reliable and scalable approach for studying lysosomal ion channel pharmacology, including TRPML1.