Automated Patch Clamp recordings of Gárdos channels (KCa3.1 / KCNN4) in physiological solutions in human primary Red Blood Cells (RBC)

Automated patch-clamp technology enables highly accurate measurement of ion channel activity in human Red Blood Cells (RBC), which is essential for understanding their physiological functions. This study specifically investigates the small endogenous Gárdos channel currents (KCa3.1 / KCNN4), highlighting their key role in red blood cell physiology. The use of low-noise amplifiers is critical, as it allows recording of low amplitude currents. High resistance patch holes, achieved through small-diameter consumables, facilitate the formation of stable giga-ohm seals with the delicate RBC membranes, reducing cellular damage. The combination of these recent improvements results in reliable, low-noise recordings, thereby supporting robust analysis of ion channel function in RBC.