Measurement of TRPM8 on Sophion´s Automated Patch Clamp systems

Transient Receptor Potential Melastatin (TRPM8) can be reliably measured on Sophion´s Automated Patch Clamp (APC) systems using stably expressing Human Embryonic Kidney (HEK-203) cells. The currents responded well to pharmacological and temperature-controlled modulation. Concentration-response curves could be performed in a cumulative and non-cumulative way as well. The acquired EC₅₀ and IC₅₀ values for racemic menthol (RM), icilin, and capsazepine were reliable, reproducible, and comparable to literature values. Based on the presented data, Sophion´s medium and high-throughput systems can be successfully used to study TRPM8 channel pharmacology and drug discovery.