High-throughput automated patch clamp of lysosomal ion channels using Sophion Bioscience platforms

In recent years, the interest in lysosomal ion channels for neurodegenerative research has increased substantially. Enhancing clearance of aggregates through lysosomal activation has proposed implications in neurodegenerative diseases, such as Parkinson’s Disease, Amyotrophic Lateral Sclerosis (ALS), and Alzheimer’s disease.
Traditional whole-cell patch clamp of lysosomes is laborious and extremely specialized. Sophion Bioscience has therefore developed a method for high-throughput automated patch clamp (APC) of enlarged lysosomes (LYSO-prep provided by Oria Bioscience) using the QPatch 48 and Qube 384 platforms. This APC method involves using small-hole, high-resistant consumables (QPlate HiR and QChip Custom) for the purpose of capturing organelles and forming tight seals, which enable stable recordings of endogenous or modified lysosomal ion channels.