Optimization of automated patch-clamp technique and comparative analysis with manual patch-clamp: A case study on the TRPV1 channel

Patch-clamp technique serve as a powerful tool for precisely measuring and characterizing ion channel currents, offering critical molecular-level insights essential for drug screening and optimization. By enabling a deeper understanding of ion channel behavior, these techniques significantly accelerate the process of drug discovery and development. In recent years, automated patch-clamp technique has undergone substantial advancements, surpassing traditional manual methods with its high throughput, improved data consistency, and automation. However, fully harnessing these advantages requires meticulous optimization of experimental conditions tailored to specific targets. Without such refinement, the high cost of consumables and operational expenses could severely hinder the widespread adoption of this technique. This study focused on the TRPV1 channel, detailing the establishment of an automated patch-clamp detection system for TRPV1 currents, optimization of experimental parameters, and a comparative evaluation of the results against manual patch-clamp techniques.

Keywords: Q2 2025