Toxin pharmacology and MoA data against Nav1.X channels: Multi-parameter analysis using Sophion Qube high-throughput automated patch clamp platform

The adaptive voltage protocol on the Sophion Qube has been used to successfully generate pharmacology for a range of small molecules and toxins, with potency values in line with the literature. Differences in specific IC50 values between this data and published data may be explained by differences in voltage protocols, particularly in the test pulse potential and length of the inactivation period, or use of different cell types. Multi-parameter analysis of Nav1.X current traces allows mechanism of action data, including state-dependence, to be generated from single-well recordings. Additionally, the expected single amino acid substitution in the voltage-sensing domain (3,6). All data presented was obtained from a single Qube experiment for each cell line tested, demonstrating the high quantity and quality of meaningful data which can be obtained using automated patch clamp technology.