Short compound exposure times for fast-desensitizing ion channels recorded on QPatch Compact

The nicotinic acetylcholine receptor alpha1 (nAChR α1) is a fast-desensitizing channel that makes the development of a stable high-throughput assay on automated patch clamp systems challenging.

• The solution stacking feature on QPatch Compact (QPC) reduces ligand-exposure times below 1 s. Using this feature, it is possible to evoke stable acetylcholine signals over several agonist stimulations.
• The QPlates microfluidic flow channel architecture ensures rapid and complete solution exchange of compound and agonist and complete washout, both of which are critical for obtaining stable recordings.