hiPSC-derived cardiomyocytes: A robust and reproducible QPatch® II assay for Nav1.5 measurements


Application Report


Kadla R Rosholm, Stefania Karatsiompani, Ozhathil Lijo Cherian



The NaV1.5 channel is critical in maintaining the physiological electrical function in cardiomyocytes (CMs). Consequently, high-throughput measurements of NaV1.5 currents (INa) in hiPSC-CMs are useful e.g. in the characterization of cardiac disease phenotypes and compound testing. QPatch II has been demonstrated as a capable platform for such measurements, and here, we present a high-performance NaV1.5 assay using iCell® Cardiomyocytes (hiPSC-CMs) from FUJIFILM Cellular Dynamics with:

  • Assay success rates ~45% with more than 85% hiPSC CMs expressing INa with peak currents |INa|> 200 pA (at -30 mV)
  • Biophysical characterization with activation/inactivation potentials in agreement with literature values
  • Cumulative compound concentration response of the local anaesthetic agent tetracaine with IC50 values in agreement
    with literature values
Go to journal

Get in Touch

We strive to provide the best for our customers, and we are always ready to help. Please let us know if you have a question for us.

Follow us