TOPIC

hiPSC-derived cardiomyocytes: A robust and reproducible QPatch® II assay for Nav1.5 measurements

Journal

Application Report

Author(s)

Kadla R Rosholm, Stefania Karatsiompani, Ozhathil Lijo Cherian

Year

2024

The NaV1.5 channel is critical in maintaining the physiological electrical function in cardiomyocytes (CMs). Consequently, high-throughput measurements of NaV1.5 currents (INa) in hiPSC-CMs are useful e.g. in the characterization of cardiac disease phenotypes and compound testing. QPatch II has been demonstrated as a capable platform for such measurements, and here, we present a high-performance NaV1.5 assay using iCell® Cardiomyocytes (hiPSC-CMs) from FUJIFILM Cellular Dynamics with:

  • Assay success rates ~45% with more than 85% hiPSC CMs expressing INa with peak currents |INa|> 200 pA (at -30 mV)
  • Biophysical characterization with activation/inactivation potentials in agreement with literature values
  • Cumulative compound concentration response of the local anaesthetic agent tetracaine with IC50 values in agreement
    with literature values
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