hCav1.2 recordings on Qube 384 using step, train and CiPA protocols

The development of screening assays for the Ca_V1.2 channel has been challenging due to the tendency of Ca_V1.2 expressing cell lines to exhibit declining current levels (rundown) during the experiment^1,2. Here we report a robust Ca_V1.2 assay yielding high success rates, low rundown and reliable pharmacology.

Pharmacology and current-voltage relationship in accordance with literature values

Success rates up to 94%

Stable currents with rundown as low as (1.2 ± 0.9)% per minute

The CiPA protocol yields stable currents with rundown as low as (1 ± 1)% per minute