Electrophysiological characterization of Icrac in rat basophilic leukemia cells (RBL-2H3) using Automated Patch Clamp

Rat basophilic leukaemia (RBL) cells endogenously express calcium-release-activated-calcium (CRAC) channels (1). CRAC channels are activated by depletion of intracellular calcium
stores via the involvement of STIM-1 (stromal interaction molecule) sensing the depletion of the stores and travelling to the cell membrane activating the channel (2, 3). Several approaches can be used to deplete calcium stores, ultimately leading to activation of ICRAC. The present work was to develop assays using different depletion strategies on the
QPatch automated patch clamp platform.