Electrophysiological characterization of Icrac in rat basophilic leukemia cells (RBL-2H3) using Automated Patch Clamp


Calcium Signaling Gordon Research Conference


Daniel Sauter, Rasmus Jacobsen



Rat basophilic leukaemia (RBL) cells endogenously express calcium-release-activated-calcium (CRAC) channels (1). CRAC channels are activated by depletion of intracellular calcium
stores via the involvement of STIM-1 (stromal interaction molecule) sensing the depletion of the stores and travelling to the cell membrane activating the channel (2, 3). Several approaches can be used to deplete calcium stores, ultimately leading to activation of ICRAC. The present work was to develop assays using different depletion strategies on the
QPatch automated patch clamp platform.

Go to journal

Get in Touch

We strive to provide the best for our customers, and we are always ready to help. Please let us know if you have a question for us.

Follow us