CHO-hNav1.7 assay on Qube pharmacology, biophysical characterization and the effect of internal fluoride

Na_V1.7 has moved into the focus of the drug discovery industry in the last years as numerous studies provided strong proof for the efficacy to alleviate both neuropathic and inflammatory pain (Sun, Cohen, & Dehnhardt, 2014). We demonstrate high reproducibility and precision of Na_V1.7 data recorded on Qube.

• 95% of all tested cells had a GΩ seal in the beginning, and 61% at the end of a long, 70 min experiment
• V_1/2 is stable over time with only a slight shift
• Experiments can be performed in physiological ringers. However, fluoride in the internal solution significantly increases success rate without any altering biophysical parameters.