Automated Patch Clamp assay for proton-activated HOTOP1 channel

Manual patch clamp is the gold standard for ion channel studies but is low-throughput and labor-intensive. Automated patch clamp (APC) systems, like the 8-well QPatch Compact (QPC) with rapid solution exchange capabilities, overcome these limitations, enabling efficient study of channels. Central to the QPC’s functionality is a microfluidic flow channel that ensures precise and controlled delivery of solutions. This microfluidic design facilitates complete and rapid solution exchange around the cell, a critical feature for accurately studying channels activated by transient stimuli such as protons. OTOP1 is a proton-selective ion channel crucial for acid sensing in tissues like the inner ear and taste buds, acting as a sour taste receptor and ammonium sensor. Characterising OTOP1’s biophysical properties is vital to understanding its physiological roles and therapeutic potential.

ICMS2025 UK