Stem Cells in Neuroscience

We are happy to sponsor the Stem Cells in Neuroscience in Tübingen, Germany.

Time:      March 11 – 13, 2024

Venue:    Institute of Neuroanatomy and Developmental Biology, Österbergstraße 3, 72074 Tübingen, Germany

From Sophion, you can meet Senior Research Scientist Kadla R Rosholm and Application Scientist Stefania Karatsiompani.

During the meeting Kadla R Rosholm will present a poster titled; Electrophysiological characterization of iPSC derived excitatory neurons, including a comparison to genetically modified Frontotemporal Dementia neuron model, using automated patch clamp.


Induced pluripotent stem cells (iPSCs) show great potential for the generation and characterization of neuronal subtypes as well as the investigation of neurological disease models. However, in practice the intercellular variability in a population of iPSC-derived neurons in combination with the low-throughput nature of manual electrophysiological experiments, have made such studies challenging.

Here we use automated patch clamp (APC) for high-throughput characterization and comparison of commercially available apparently healthy normal (WT) and frontotemporal dementia (FTD; genetically engineered Granulin R493X heterozygous knockout) iPSC derived excitatory neurons. The results include an optimization of the cell suspension for APC and an analysis of voltage-gated (Kv and Nav) and ligand-gated (AMPA) ion channel currents and how they develop over time. We also compare action potential parameters (such as spike frequency, spike threshold and action potential amplitude) in WT and FTD model iPSC-derived excitatory neurons.



Read about the meeting here
Read about our work with stem cells & APC
Contact us for more information