Publications - Sophion

Voltage- and current clamp on induced pluripotent cardiomyocytes with automated patch clamp

Author(s): Daniel Sauter, Anders Lindqvist, Rasmus Bjørn Jacobsen, Jens Henneke, Mads P.G. Korsgaard

Use- and state-dependent Nav1.5 blockers on QPatch X and in vivo and in vitro assays

Author(s): M Knirke Jensen, Tomoko Sakakura, Yasuyuki Abe, Hideo Takamori, Kiyoshi Takasuna, Yuji Tsurubuchi, Morten Sunesen

Upscaling and automation of electrophysiology: toward high throughput screening in ion channel drug discovery

Author(s): M Asmild, N Oswald, KM Krzywkowski, S Friis, RB Jacobsen, D Reuter, R Taboryski, J Kutchinsky, RK Vestergaard, RL Schrøder, CB Sørensen, M Bech, MP Korsgaard, NJ Willumsen

TRP’ing in multi-hole mode

Author(s): Rasmus B Jacobsen, Hervør L Olsen

Transient expression of the NMDA receptor in HEK cells co-expressing the glutamate transporter for the screening of potential positive allosteric modulators of NR2B

Author(s): Michael J Morton, John Malone, Paul Sharpe, Graham Sproat, Malcolm Haddrick, Joe Mather

TMEM16A on Qube 384

Author(s): Joseph G. McGivern*, John K. Sullivan*, Kathryn A. Henckels*, Paul Wang*, David Powers*, Daniel Sauter**, Lars Løjkner**, Rasmus Jakobsen**, Mads Korsgaard**

The E15R Point Mutation in Scorpion Toxin Cn2 Uncouples Its Depressant and Excitatory Activities on Human NaV1.6

Author(s): Mathilde R. Israel, Panumart Thongyoo, Jennifer R. Deuis, David J. Craik, Irina Vetter, Thomas Durek

Temperature effect on hERG channel pharmacology measured using the Qube automated patch clamp system

Author(s): Anders Lindqvist, Mette Christensen, Rasmus Jacobsen, Göran Mattson

Synthesis and biological evaluation of a series of multi-target N-substituted cyclic imide derivatives with potential antipsychotic effect

Author(s): Mingshuo Xu, Yu Wang, Feipu Yang, Chunhui Wu, Zhen Wang, Bin Ye, Xiangrui Jiang, Qingjie Zhao, JianfengLi, Yongjian Liu, Junchi Zhang, Guanghui Tian, Yang He, Jingshan Shen, Hualiang Jiang

Strategies for enhancing throughput in ion channel drug screening

Author(s): Rasmus B. Jacobsen, Naja M. Sørensen, Hervør L. Olsen, M. Sunesen, *Niels J. Willumsen

Sophion analyzer as a tool for biophysical and pharmacological characterization of eight Nav subtypes evaluated in parallel on Qube

Author(s): Mads P. G. Korsgaard, Lars Damgaard Løjkner, Anders Lindqvist, Mette T Christensen, Thomas Binzer

Simple and Effective Generation of Cell-based Assays for Ion Channels, Transporters, and Kinase Screening in Biologically Relevant Cells Using Scalable Transient Transfection

Author(s): Qin (Ching) Chen, James Brady, Karen Donato, Meg Duskin, and Madhusudan Peshwa

Scaffolds within scaffolds: Generating ion channel blocking antibodies by fusing knottin to peripheral CDR loops

Author(s): A.Karatt-Vellatt1, S. Surade1, D. C. Bell1, T. Leutkens1, E. Masters1, A.M. Luther1, N.M. Sørensen2, N. Butt1 & J. McCafferty1

Rapid generation of cells for ion channels assays: Efficient, large scale transfection using the MaxCyte STX

Author(s): James Brady, Peer Heine, Rama Shivakumar, Angelia Viley, Madhusudan Peshwa, Karen Donato and Krista Steger - MaxCyte Mark Rothenberg. Corning Inc., Kennebunk, ME, USA. Hervør Lykke Olsen, Nikolaj Nielsen, Kristina Christensen, Jeffrey Webber and Morten Sunesen - Sophion Bioscience

Qube as a tool for assay optimization of CiPA cells and protocols by using multiple IC, EC solutions and hERG, Nav1.5 and Cav1.2 on the same QChip

Author(s): Melanie Schupp, Anders Lindqvist, Pia Bladsgaard Andersen, Weifeng Yu

Qube 384-channel patch clamp characterization of the liquid flow channels

Author(s): Rasmus B Jacobsen, Søren Friis, Hervør L Olsen, Mette T Christensen, Kristina M Christensen, Peder S Pedersen, Anders Hyldgaard, Morten Sunesen, Anders Lindqvist

Qube 384 channel patch clamp screening system ligand gated ion channel applications

Author(s): Søren Friis, Rasmus B Jacobsen, Hervør Lykke Olsen, Mette T Christensen, Kristina M. Christensen, Peder Skafte Pedersen, Lasse Homann, Morten Sunesen, Anders Lindqvist

Preparation, Antiepileptic Activity, and Cardiovascular Safety of Dihydropyrazoles as Brain-Penetrant T-Type Calcium Channel Blockers

Author(s): Lubos Remen†, Olivier Bezençon*†, Lloyd Simons‡, Rick Gaston‡, Dennis Downing‡, John Gatfield†, Catherine Roch†, Melanie Kessler†, Johannes Mosbacher†, Thomas Pfeifer†, Corinna Grisostomi†, Markus Rey†, Eric A. Ertel†, and Richard Moon†

Pharmacological characterization of voltage- and ligand-gated ion channels

Author(s): Hervør L Olsen, Rasmus B Jacobsen, Søren Friis, Morten Sunesen, Niels Willumsen, Rikke Schrøder, Naja M Sørensen, M Knirke Jensen

Parallel patch clamp of alpha 7 nicotinic acetylcholine receptor channels

Author(s): Søren Friis, Morten Sunesen, Chris Mathes, Mark Bowlby, John Dunlop, Niels Willumsen

Optimizing CHO hERG for automated patch clamping

Author(s): Søren Friis, Hervør L Olsen, Jeffrey Webber, M Knirke Jensen, Dorthe Nielsen, Morten Sunesen, Chris Mathes

Optical modulation of ion channels

Author(s): Kim Boddum, Peder Skafte-Pedersen, Jens Henneke, Daniel Sauter, Jiaye Zhang, Sandra Wilson

Optical modulation of ion channels

Author(s): Kim Boddum, Peder Skafte-Pedersen, Jens Henneke, Daniel Sauter, Jiaye Zhang, Sandra Wilson

NMDA Receptor Modulators in QPatch

Author(s): Ezio Bettini, Laura Aldegheri, Laura Castelletti, Mauro Corsi and Caterina Virginio

NaV1.5 big late : An inactivation deficient mutant of NaV1.5 as screening tool for late sodium currents of the cardiac action potential

Author(s): Camille Bouyer, Simon Hebeisen

HT Automation for patch clamp based primary screen for Nav1.1 using Qube 384

Author(s): Julie K. Klint, Melanie Schupp, Daniel R P Sauter, Rasmus Jacobsen, Thomas Binzer

High Throughput Electrophysiology: New Perspectives for Ion Channel Drug Discovery

Author(s): Niels J. Willumsen, Morten Bech, Søren-Peter Olesen, Bo Skaaning Jensen, Mads P. G. Korsgaard, and Palle Christophersen

hCav1.2 recordings using QPatch

Author(s): Kadla R Rosholm, Melanie Schupp

GI-530159, a novel, selective, mechanosensitive two-pore-domain potassium (K2P ) channel opener, reduces rat dorsal root ganglion neuron excitability

Author(s): Loucif AJC, Saintot PP, Liu J, Antonio BM, Zellmer SG, Yoger K, Veale EL, Wilbrey A, Omoto K, Cao L, Gutteridge A, Castle NA, Stevens EB, Mathie A

GABAA receptor pharmacology evaluted in overexpressing HEK cells and primary astrocytes on QPatch

Author(s): Kim Boddum, Kadla Røskva Rosholm, Linda Blomster, Hervør Lykke Olsen, Naja Møller Sørensen, Göran Mattsson

GABA-A receptor pharmacology evaluated in overexpressing HEK cells and primary astrocytes on QPatch

Author(s): Kim Boddum1, Kadla Røskva Rosholm1, Linda Blomster2, Hervør Lykke Olsen, Naja Møller Sørensen1, Göran Mattsson1

Exploring stem cell-derived cardiomyocytes with automated patch clamp techniques

Author(s): Rikke P Schrøder, Mette T Christensen, Blake Anson, Morten Sunesen

Evaluation of throughput and data quality

Author(s): Hervor L Olsen, Rikke P Schrøder, Rasmus B Jacobsen, Søren Friis, Morten Sunesen

Enhancing throughput with multiple cell lines per well with the QPatch HTX

Author(s): Hervor L Olsen, Rikke P Schrøder

Enhancing throughput with multiple cell lines per well

Author(s): Hervor L Olsen, Dorthe Nielsen, Morten Sunesen

Endogenous Ion Channels of Mammalian Cell Lines Characterization

Author(s): Søren Friis, Rasmus B Jacobsen, Morten Sunesen, Dorthe Nielsen, Nadia Larsen, Chris Mathes, Niels Willumsen

Electrophysiological properties of iCell cardiomyocytes obtained by automated patch clamp

Author(s): Rikke P Schrøder 1), Mette T Christensen 1), Giorgia Salvagiotto 2), Blake Anson 2), Morten Sunesen 1)

Electrophysiological characterization of human induced pluripotent stem cell-derived dopaminergic neurons using manual and automated patch clamp systems

Author(s): Denise Franz, Jan Giemsa, Oliver Klink, Hervør Lykke Olsen

Direct evidence for high affinity blockade of NaV1.6 channel subtype by huwentoxin-IV spider peptide, using multiscale functional approaches

Author(s): Tânia C. Gonçalves, Rachid Boukaiba, Jordi Molgób, Muriel Amar, Michel Partiseti, Denis Servent, Evelyne Benoit

Development of high-throughput electrophysiological assay for the screening of hERG ion channel modulators using Sophion Qube 384

Author(s): David J. Standing, Kelly Gatfield, David L. Downie, Metul Patel, Andrew J. Brown

Development of an Ultra High-Throughput Screen to Identify Antagonists for GluK1

Author(s): Kelli Solly1 , Carissa Quinn1 , Rebecca Klein2 , Darrel Henze 2 , Eric Johnson1 , and Michael Finley 1

Development of a novel screening system to identify activators to two-pore domain potassium channels (K2Ps)

Author(s): David McCoull, Emma Ococks, Paul D Wright

Data quality is not affected by cell suspension density

Author(s): Chris Mathes, Hervør L Olsen, Michael Stoltenborg, Morten Sunesen, Niels Willumsen

Chemoselective tarantula toxins report voltage activation of wild-type ion channels in live cells

Author(s): Drew C. Tilleya (1, Kenneth S. Euma, (b,1,2, Sebastian Fletcher-Taylora, Daniel C. Austina, Christophe Dupréb, Lilian A. Patrónb, Rita L. Garciac, Kit Lamd, Vladimir Yarov-Yarovoya,d, Bruce E. Cohenc, and Jon T. Sacka,b (3

Characterization of human iPSC-derived cardiomyocytes (Cor.4U) on an automated planar patch clamp set-up (QPatch)

Author(s): Daniel Sauter, Kim Boddum, Kazuya Tsurudome, Rasmus B Jacobsen, Göran Mattsson

Characterization of hERG blockers using the automated QPatch 16 screening system

Author(s): Rasmus B Jacobsen, Simon Pedersen, Søren Friis, Nicoline Andersen, Niels Willumsen

Biophysical and pharmacological profiling of multiple Nav subtypes on QPatch HT

Author(s): Anders Lindqvist, Søren Friis, Mette T. Christensen & Mads P.G. Korsgaard

Biophysical and pharmacological characterization of multiple Nav subtypes on Qube

Author(s): Anders Lindqvist, Søren Friis, Mette T. Christensen & Mads P.G. Korsgaard

Biophysical and pharmacological characterization of ligandgated ion channels in multi-hole mode

Author(s): M. Knirke Jensen, Rikke Schrøder, Hervør L. Olsen, Rasmus B. Jacobsen, Jeffrey Webber, Søren Friis, Dorthe Nielsen, Mette T. Christensen, Morten Sunesen

Automation of patch clamp techniques: Technical validation through characterization of voltage-gated potassium channels and ligand-gated ion channels

Author(s): Karen Krzywkowski, Rikke Schrøder, Trine Ljungstrøm, Jonatan Kutchinsky, Søren Friis, Margit Asmild, Rafael Taborski, Simon Pedersen, Ras K. Vestergaard, Rasmus B Jacobsen, Nathalie Helix, Claus B Sørensen Morten Bech, Jeppe K Christensen, Simon du Beau, Niels Willumsen

Automated Patch-Clamp Technique: Increased Throughput in Functional Characterization and in Pharmacological Screening of Small-Conductance Ca2+ Release-Activated Ca2+ Channels

Author(s): Rikke L. Schrøder 1), Søren Friis 1), Morten Sunesen 1), Chris Mathes 2), and Niels J. Willumsen 3)

Assessing functional properties of ligand-gated ion channels with automated whole-cell patch clamp technology

Author(s): Hervør L Olsen, Rasmus B Jacobsen, Rikke Schrøder, Jonatan Kutchinsky, Morten Sunesen, Niels Willumsen

Are stem-cell cardiomyocytes a viable cellular reagent for automated patch-clamp?

Author(s): Michael Morton, Lauren Eades

Application of QPatch 16 for drug screening of ligand-gated ion channels

Author(s): Nicoline R. Andersen, Simon Pedersen, Jeppe K. Christensen, Michael Stoltenborg, Morten Sunesen, Niels J. Willumsen

“Clip Detecting” with series resistance compensation using an automated patch clamp system

Author(s): Lars Damgaard Løjkner, Chris Gibbs, Jesper Gerved, Jens Henneke, Jonatan Kutchinsky, Rasmus B Jacobsen, Morten Sunesen