Publications - Sophion

Wnt peptides control mammalian cancer cell membrane potential

Author(s): Jonathan Ashmore, Hervør Olsen, Naja Sørensen, Chris Thrasivoulou , Aamir Ahmed

Voltage- and current clamp on induced pluripotent cardiomyocytes with automated patch clamp

Author(s): Daniel Sauter, Anders Lindqvist, Rasmus Bjørn Jacobsen, Jens Henneke, Mads P.G. Korsgaard

Verification of CiPA Recommended Voltage Protocols in Patch-Clamp Assay for hERG, Cav1.2 and Late Nav1.5 Currents

Author(s): Yoshimi KATAYAMA, Hiroshi MATSUKAWA, Tomokazu KANEHISA, Ayane ABE, Takashi YOSHINAGA, Keiichi ASAKURA, Kimihito YOSHIKAWA, Kazuya TSURUDOME, Tadaaki TSUKAMOTO

Use- and state-dependent Nav1.5 blockers on QPatch X and in vivo and in vitro assays

Author(s): M Knirke Jensen, Tomoko Sakakura, Yasuyuki Abe, Hideo Takamori, Kiyoshi Takasuna, Yuji Tsurubuchi, Morten Sunesen

Upscaling and automation of electrophysiology: toward high throughput screening in ion channel drug discovery

Author(s): M Asmild, N Oswald, KM Krzywkowski, S Friis, RB Jacobsen, D Reuter, R Taboryski, J Kutchinsky, RK Vestergaard, RL Schrøder, CB Sørensen, M Bech, MP Korsgaard, NJ Willumsen

TRP’ing in multi-hole mode

Author(s): Rasmus B Jacobsen, Hervør L Olsen

Transient expression of the NMDA receptor in HEK cells co-expressing the glutamate transporter for the screening of potential positive allosteric modulators of NR2B

Author(s): Michael J Morton, John Malone, Paul Sharpe, Graham Sproat, Malcolm Haddrick, Joe Mather

TMEM16A on Qube 384

Author(s): Joseph G. McGivern*, John K. Sullivan*, Kathryn A. Henckels*, Paul Wang*, David Powers*, Daniel Sauter**, Lars Løjkner**, Rasmus Jakobsen**, Mads Korsgaard**

Temperature effect on hERG channel pharmacology measured using the Qube automated patch clamp system

Author(s): Anders Lindqvist, Mette Christensen, Rasmus Jacobsen, Göran Mattson

Structure-based identification of novel KNa1.1 inhibitors

Author(s): Bethan A. Cole, Rachel M. Johnson, Hattapark Dejakaisaya, Stephen P. Muench, & Jonathan D. Lippiat

Strategies for enhancing throughput in ion channel drug screening

Author(s): Rasmus B. Jacobsen, Naja M. Sørensen, Hervør L. Olsen, M. Sunesen, *Niels J. Willumsen

Sophion analyzer as a tool for biophysical and pharmacological characterization of eight Nav subtypes evaluated in parallel on Qube

Author(s): Mads P. G. Korsgaard, Lars Damgaard Løjkner, Anders Lindqvist, Mette T Christensen, Thomas Binzer

Simultaneous Measurement of Cardiac Nav1.5 Peak and Late Currents in an Automated QPatch Platform

Author(s): Donglin Guo, Sunny Sun and Stephen Jenkinson

Simultaneous Measurement of Cardiac hERG and Nav1.5 Currents Using an Automated Qube® Patch Clamp Platform

Author(s): Donglin Guo, Tiffany Lee, Daniel Sauter and Stephen Jenkinson

Simple and Effective Generation of Cell-based Assays for Ion Channels, Transporters, and Kinase Screening in Biologically Relevant Cells Using Scalable Transient Transfection

Author(s): Qin (Ching) Chen, James Brady, Karen Donato, Meg Duskin, and Madhusudan Peshwa

Scaffolds within scaffolds: Generating ion channel blocking antibodies by fusing knottin to peripheral CDR loops

Author(s): A.Karatt-Vellatt1, S. Surade1, D. C. Bell1, T. Leutkens1, E. Masters1, A.M. Luther1, N.M. Sørensen2, N. Butt1 & J. McCafferty1

Rapid generation of cells for ion channels assays: Efficient, large scale transfection using the MaxCyte STX

Author(s): James Brady, Peer Heine, Rama Shivakumar, Angelia Viley, Madhusudan Peshwa, Karen Donato and Krista Steger - MaxCyte Mark Rothenberg. Corning Inc., Kennebunk, ME, USA. Hervør Lykke Olsen, Nikolaj Nielsen, Kristina Christensen, Jeffrey Webber and Morten Sunesen - Sophion Bioscience

Qube as a tool for assay optimization of CiPA cells and protocols by using multiple IC, EC solutions and hERG, Nav1.5 and Cav1.2 on the same QChip

Author(s): Melanie Schupp, Anders Lindqvist, Pia Bladsgaard Andersen, Weifeng Yu

Qube 384-channel patch clamp characterization of the liquid flow channels

Author(s): Rasmus B Jacobsen, Søren Friis, Hervør L Olsen, Mette T Christensen, Kristina M Christensen, Peder S Pedersen, Anders Hyldgaard, Morten Sunesen, Anders Lindqvist

Qube 384 channel patch clamp screening system ligand gated ion channel applications

Author(s): Søren Friis, Rasmus B Jacobsen, Hervør Lykke Olsen, Mette T Christensen, Kristina M. Christensen, Peder Skafte Pedersen, Lasse Homann, Morten Sunesen, Anders Lindqvist

Preparation, Antiepileptic Activity, and Cardiovascular Safety of Dihydropyrazoles as Brain-Penetrant T-Type Calcium Channel Blockers

Author(s): Lubos Remen†, Olivier Bezençon*†, Lloyd Simons‡, Rick Gaston‡, Dennis Downing‡, John Gatfield†, Catherine Roch†, Melanie Kessler†, Johannes Mosbacher†, Thomas Pfeifer†, Corinna Grisostomi†, Markus Rey†, Eric A. Ertel†, and Richard Moon†

Predicting Cardiac Proarrhythmic Risk Exclusively Using Automated Patch Clamp Data

Author(s): Edward SA Humphries, John Ridley, Robert Kirby and Marc Rogers

Pharmacological characterization of voltage- and ligand-gated ion channels

Author(s): Hervør L Olsen, Rasmus B Jacobsen, Søren Friis, Morten Sunesen, Niels Willumsen, Rikke Schrøder, Naja M Sørensen, M Knirke Jensen

Parallel patch clamp of alpha 7 nicotinic acetylcholine receptor channels

Author(s): Søren Friis, Morten Sunesen, Chris Mathes, Mark Bowlby, John Dunlop, Niels Willumsen

Optimizing CHO hERG for automated patch clamping

Author(s): Søren Friis, Hervør L Olsen, Jeffrey Webber, M Knirke Jensen, Dorthe Nielsen, Morten Sunesen, Chris Mathes

Optimization of Cardiac CiPA Targets (hCav1.2 and hKCNQ1/hminK) on the QPatch HT Automated System

Author(s): Bryan Koci, Muthukrishnan Renganathan, Andi Cook, Haiyang Wei, Diane Werth

Optical modulation of ion channels using Qube Opto

Author(s): Kim Boddum, Peder Skafte-Pedersen, Jens Henneke, Daniel Sauter, Jean Francois Rolland, Jiaye Zhang, Sandra Wilson

Optical modulation of ion channels

Author(s): Kim Boddum, Peder Skafte-Pedersen, Jens Henneke, Daniel Sauter, Jiaye Zhang, Sandra Wilson

NMDA Receptor Modulators in QPatch

Author(s): Ezio Bettini, Laura Aldegheri, Laura Castelletti, Mauro Corsi and Caterina Virginio

New CiPA cardiac ion channel cell lines and assays for in vitro proarrhythmia risk assessment

Author(s): Edward SA Humphries, Robert W. Kirby, Louise Webdale and Marc Rogers

NaV1.5 big late : An inactivation deficient mutant of NaV1.5 as screening tool for late sodium currents of the cardiac action potential

Author(s): Camille Bouyer, Simon Hebeisen

Multi-parameter ion channel screening: mechanism-of-action data directly from HTS

Author(s): Juha Kammonen, Anthony Lawrence and Jonathan Mann

Ligand-activation of GABAA receptors on the automated patch clamp platforms QPatch and Qube 384 using conventional electrophysiology and optopharmacology

Author(s): Melanie Schupp, Kim Boddum, Daniel R. Sauter, Peder Skafte-Pedersen, Linda Blomster, Hervør L. Olsen, Rasmus. B. Jacobsen

In vitro inflammatory knee pain: Of Mice and Men

Author(s): Sampurna Chakrabarti, Luke A. Pattison, Kaajal Singhal, Jolanta Mil, David C Bulmer, Deepak R Jadon, Ewan St. John Smith

Identification of novel activators of two-pore domain potassium (K2P) channels

Author(s): David McCoull, Lewis Byrom, Jonathan Large, Jeff Jerman, Paul D Wright

HT Automation for patch clamp based primary screen for Nav1.1 using Qube 384

Author(s): Julie K. Klint, Melanie Schupp, Daniel R P Sauter, Rasmus Jacobsen, Thomas Binzer

High-throughput screening of induced pluripotent stem cell-derived motor neurons on Qube and QPatch

Author(s): Kadla Røskva Rosholm, Daniel Sauter, Melanie Schupp, Rasmus B Jacobsen

High Throughput Electrophysiology: New Perspectives for Ion Channel Drug Discovery

Author(s): Niels J. Willumsen, Morten Bech, Søren-Peter Olesen, Bo Skaaning Jensen, Mads P. G. Korsgaard, and Palle Christophersen

Hesperetin preferentially inhibits slow-activating currents of an LQT3 syndrome Na+ channel mutation

Author(s): Julio Alvarez-Collazo, Alejandro López-Requena, Loipa Galán, Ariel Talavera, Julio L. Alvarez and Karel Talavera

GI-530159, a novel, selective, mechanosensitive two-pore-domain potassium (K2P ) channel opener, reduces rat dorsal root ganglion neuron excitability

Author(s): Loucif AJC, Saintot PP, Liu J, Antonio BM, Zellmer SG, Yoger K, Veale EL, Wilbrey A, Omoto K, Cao L, Gutteridge A, Castle NA, Stevens EB, Mathie A

Generating potent and selective inhibitors of Kv1.3 ion channels by fusing venom derived mini proteins into peripheral CDR loops of antibodies

Author(s): Lien Moreels, A. Karatt-Vellatt, D. C. Bell, S. Surade, P. A. Slavny, T. Luetkens, E. Masters & J. McCafferty

GABAA receptor pharmacology evaluted in overexpressing HEK cells and primary astrocytes on QPatch

Author(s): Kim Boddum, Kadla Røskva Rosholm, Linda Blomster, Hervør Lykke Olsen, Naja Møller Sørensen, Göran Mattsson

GABA-A receptor pharmacology evaluated in overexpressing HEK cells and primary astrocytes on QPatch

Author(s): Kim Boddum1, Kadla Røskva Rosholm1, Linda Blomster2, Hervør Lykke Olsen, Naja Møller Sørensen1, Göran Mattsson1

Functional Assessment of hNav1.x Ion Channels Using State- Dependent Protocols on the QPatch HT Automated Patch Clamp System

Author(s): Bryan Koci, Jennifer Wesley, Muthukrishnan Renganathan, Haiyang Wei, Diane Werth

Exploring stem cell-derived cardiomyocytes with automated patch clamp techniques

Author(s): Rikke P Schrøder, Mette T Christensen, Blake Anson, Morten Sunesen

Evaluation of throughput and data quality

Author(s): Hervor L Olsen, Rikke P Schrøder, Rasmus B Jacobsen, Søren Friis, Morten Sunesen

Estimating hERG drug binding using temperature-controlled high throughput automated patch clamp

Author(s): Anders Lindqvist, Mette T Christensen

Enhancing throughput with multiple cell lines per well with the QPatch HTX

Author(s): Hervor L Olsen, Rikke P Schrøder

Enhancing throughput with multiple cell lines per well

Author(s): Hervor L Olsen, Dorthe Nielsen, Morten Sunesen

Endogenous Ion Channels of Mammalian Cell Lines Characterization

Author(s): Søren Friis, Rasmus B Jacobsen, Morten Sunesen, Dorthe Nielsen, Nadia Larsen, Chris Mathes, Niels Willumsen

Electrophysiological properties of iCell cardiomyocytes obtained by automated patch clamp

Author(s): Rikke P Schrøder 1), Mette T Christensen 1), Giorgia Salvagiotto 2), Blake Anson 2), Morten Sunesen 1)

Electrophysiological characterization of human induced pluripotent stem cell-derived dopaminergic neurons using manual and automated patch clamp systems

Author(s): Denise Franz, Jan Giemsa, Oliver Klink, Hervør Lykke Olsen

Electrophysiological characterization of human dopaminergic neurons derived from LUHMES cells

Author(s): Udo Kraushaar, Dominik Loser, Timm Danker, Clemens Möller, Marcel Leist

Development of high-throughput electrophysiological assay for the screening of hERG ion channel modulators using Sophion Qube 384

Author(s): David J. Standing, Kelly Gatfield, David L. Downie, Metul Patel, Andrew J. Brown

Development of an Ultra High-Throughput Screen to Identify Antagonists for GluK1

Author(s): Kelli Solly1 , Carissa Quinn1 , Rebecca Klein2 , Darrel Henze 2 , Eric Johnson1 , and Michael Finley 1

Development of a novel screening system to identify activators to two-pore domain potassium channels (K2Ps)

Author(s): David McCoull, Emma Ococks, Paul D Wright

Development and validation of NMDA ligand-gated ion channel assays using the Qube 384 automated electrophysiology platform

Author(s): Abigail Marklew, Juha Kammonen, Emma Richardson, and Jonathan Mann

Development and validation of ligand-gated ion channel assays using the Qube 384 automated electrophysiology platform

Author(s): Abigail Marklew, Juha Kammonen, Emma Richardson and Gary Clark

Data quality is not affected by cell suspension density

Author(s): Chris Mathes, Hervør L Olsen, Michael Stoltenborg, Morten Sunesen, Niels Willumsen

Chemoselective tarantula toxins report voltage activation of wild-type ion channels in live cells

Author(s): Drew C. Tilleya (1, Kenneth S. Euma, (b,1,2, Sebastian Fletcher-Taylora, Daniel C. Austina, Christophe Dupréb, Lilian A. Patrónb, Rita L. Garciac, Kit Lamd, Vladimir Yarov-Yarovoya,d, Bruce E. Cohenc, and Jon T. Sacka,b (3

Characterization of Human iPSC-derived cardiomyocytes (Cor.4U®) on an automated planar patch clamp set up (Qube)

Author(s): Daniel R. Sauter, Kadla R. Rosholm, Kim Boddum, Kazuya Tsurudome, Rasmus. B. Jacobsen, Göran Mattsson

Characterization of human iPSC-derived cardiomyocytes (Cor.4U) on an automated planar patch clamp set-up (QPatch)

Author(s): Daniel Sauter, Kim Boddum, Kazuya Tsurudome, Rasmus B Jacobsen, Göran Mattsson

Characterization of hiPSC-derived neurological disease models using automated patch clamp (Qube and QPatch)

Author(s): Kadla Røskva Rosholm, Melanie Schupp, Daniel Sauter, Rasmus B Jacobsen

Characterization of hiPSC-derived neurological disease models using automated patch clamp (Qube and QPatch)

Author(s): Kadla Røskva Rosholm, Melanie Schupp, Daniel Sauter, Rasmus B Jacobsen

Characterization of hERG blockers using the automated QPatch 16 screening system

Author(s): Rasmus B Jacobsen, Simon Pedersen, Søren Friis, Nicoline Andersen, Niels Willumsen

Biophysical and pharmacological profiling of multiple voltage-gated sodium channel subtypes on QPatch II

Author(s): Daniel Sauter, Mette T Christensen, Rasmus B Jacobsen, Göran Mattsson

Biophysical and pharmacological profiling of multiple voltage-gated sodium channel subtypes on QPatch II

Author(s): Daniel Sauter, Mette T Christensen, Rasmus B Jacobsen, Göran Mattsson

Biophysical and pharmacological profiling of multiple Nav subtypes on QPatch HT

Author(s): Anders Lindqvist, Søren Friis, Mette T. Christensen & Mads P.G. Korsgaard

Biophysical and pharmacological characterization of multiple Nav subtypes on Qube

Author(s): Anders Lindqvist, Søren Friis, Mette T. Christensen & Mads P.G. Korsgaard

Biophysical and pharmacological characterization of ligandgated ion channels in multi-hole mode

Author(s): M. Knirke Jensen, Rikke Schrøder, Hervør L. Olsen, Rasmus B. Jacobsen, Jeffrey Webber, Søren Friis, Dorthe Nielsen, Mette T. Christensen, Morten Sunesen

Automation of patch clamp techniques: Technical validation through characterization of voltage-gated potassium channels and ligand-gated ion channels

Author(s): Karen Krzywkowski, Rikke Schrøder, Trine Ljungstrøm, Jonatan Kutchinsky, Søren Friis, Margit Asmild, Rafael Taborski, Simon Pedersen, Ras K. Vestergaard, Rasmus B Jacobsen, Nathalie Helix, Claus B Sørensen Morten Bech, Jeppe K Christensen, Simon du Beau, Niels Willumsen

Automatic estimation of hNaV1.5 channel inactivation improves pharmacological evaluation using the new adaptive protocol feature on Qube

Author(s): Anders Lindqvist

Automated Patch-Clamp Technique: Increased Throughput in Functional Characterization and in Pharmacological Screening of Small-Conductance Ca2+ Release-Activated Ca2+ Channels

Author(s): Rikke L. Schrøder 1), Søren Friis 1), Morten Sunesen 1), Chris Mathes 2), and Niels J. Willumsen 3)

Automated High Throughput Na+ Late Current Assay on QPatch HT Platform for CiPA28

Author(s): Muthukrishnan Renganathan, Bryan J. Koci, Andi Cook, Haiyang Wei, Diane Werth

Assessing functional properties of ligand-gated ion channels with automated whole-cell patch clamp technology

Author(s): Hervør L Olsen, Rasmus B Jacobsen, Rikke Schrøder, Jonatan Kutchinsky, Morten Sunesen, Niels Willumsen

Are stem-cell cardiomyocytes a viable cellular reagent for automated patch-clamp?

Author(s): Michael Morton, Lauren Eades

Application of QPatch 16 for drug screening of ligand-gated ion channels

Author(s): Nicoline R. Andersen, Simon Pedersen, Jeppe K. Christensen, Michael Stoltenborg, Morten Sunesen, Niels J. Willumsen

Adaptive voltage protocols increase precision of voltage-gated ion channel measurements on highthroughput automated patch clamp platforms

Author(s): Sarah Williams & Juha Kammonen

Activities for Optimizing CiPA Recommended Protocols in Patch-Clamp Assay

Author(s): Yoshimi KATAYAMA, Hiroshi MATSUKAWA, Tomokazu KANEHISA, Ayane ABE, Takashi YOSHINAGA, Keiichi ASAKURA, Kimihito YOSHIKAWA, Kazuya TSURUDOME, Tadaaki TSUKAMOTO

A pharmacological synopsis of small molecules, toxins and CiPA compounds targeting human cardiac Kv4.3 channels

Author(s): Brigitte Schombert, Camille Sanson, Sylvie Houtmann, Michel Partiseti and G. Andrees Böhme

“Clip Detecting” with series resistance compensation using an automated patch clamp system

Author(s): Lars Damgaard Løjkner, Chris Gibbs, Jesper Gerved, Jens Henneke, Jonatan Kutchinsky, Rasmus B Jacobsen, Morten Sunesen