ion channel Archives - Sophion
QPatch automated patch clamp

New paper on KCa2.X (SK) on QPatch

Small conductance calcium-activated K+ channels (KCa2.X, SK channels) are promising new targets for the treatment of Atrial fibrillation (AF), the most common type of arrhythmia. In a collaboration with Acesion Pharma, Bo Bentzen and his group revealed an inhibitory effect of some established AF drugs on SK channels using Sophion`s QPatch.   The paper benefits from QPatch`s capability to gain gigaohm seal resistances without relying on the presence of seal enhancing fluoride in the internal solution.

Read more here

Interested in CFTR? ….or traditional Chinese/Japanese medicine?

Another paper has just been published using QPatch, showing that the Kampo medicine Mashiningan (MNG) improves opioid-induced constipation in rats by activating cystic fibrosis transmembrane conductance regulator. The effects were determined using QPatch.

To our knowledge this is one of the first papers on traditional Japanese/Chinese medicine-based on studies with a QPatch, which we find rather exciting.

For those of you who are not acquainted with Japanese Kampo, it is based on traditional Chinese medicine but adapted to Japanese culture. Kampo is widely practiced in Japan, and is fully integrated into the modern healthcare system.

You can read more about Kampo here and read more from the paper here

High throughput screening

Internal solution exchange on Qube 384 – New application report

Internal addition of compounds targeting the chloride channel CIC-1.

We demonstrate a robust ClC-1 assay on Qube with internal addition of compounds. The assay shows biophysical characteristics as expected for ClC-1 with good pharmacology and high Z-score. We further introduce Sophion`s Analyzer software that allows rapid analysis of large data sets to answer advanced electrophysiological questions, in the present case: What is the mode of action of a novel, unknown compound?

See the full application report here