high throughput screening Archives - Sophion

High Throughput Screening on Kv1.5 using Qube 384 Mk II

The Kv1.5 channel is found in many tissues and is the molecular background behind Ikur in the heart – contributing to the heart’s repolarisation. If it does not work well, you can die. However, blocking this channel pharmacologically might treat various heart arrhythmias so, therefore, it can be beneficial to screen for that, so we have developed an HTS assay for this target. Read more here.

Eurofins Panlabs Inc. announces the upgrade to 384-format Automated Patch-Clamping

In July, Eurofins acquired a Qube 384, which was installed shortly after. As always, the selection process was lengthy and thorough to ensure that Eurofins selected the instrument that provided the best voltage clamp, success rates and unattended usage. After careful consideration, the group selected the Sophion Bioscience Qube 384. The instrument installation went smoothly, and the staff was able to handle the setup and execution of experiments after just one week of installation and training.

The Scientific Director at Eurofins, Bryan Koci, explains:

“We made a thorough comparison in the field of 384-APC’s and selected Qube 384 for its high performance and design, where everything is integrated and user-friendly. Being in the midst of unusual times because of the global impact of Covid-19, at Eurofins, our primary concern is our commitments to deliver to our clients and the safety of our employees and their families. We believe an extra benefit of the Qube 384 platform is that it can run mostly unattended, without significant intervention from the staff, allowing a safer work environment for our employees while continuing to provide uninterrupted services for clients. The Qube384 platform is an exciting addition to Eurofins Discovery’s comprehensive ion channel screening services and we are looking forward to providing high-quality, high throughput, screening data on Qube 384 to our clients in the near future.”

Qube Falcon is ready to increase your lab efficiency

Qube Falcon has been released and with that a range of upgrades to continue help finding better drugs faster. Since Eagle, Qube has had adaptive protocols which enables cell-specific recording to obtain much tighter data. Now the adaptive protocols have been more now flexible to cover the whole range from 10-90 % activation/inactivation. The cell-specific result can also be applied to both recording segments and to holding potentials.

Falcon can run idle sweep. These are the sweeps between one compound period and the next and gives more homogenous stimulation frequency of the cells and consequently compound effects.

Qube has sophisticated mechanisms to compensate for series resistance, capacitance and leak and with Falcon, we have faster computers to calculate all these parameters in parallel for all 384 amplifiers in only a little more than 4 seconds. Previously it took almost 10 seconds. The improvement means you can e.g. run CiPA hERG protocols with parameter estimation between every sweep.

Qube is a high fidelity automated patch clamp instrument meant to run unattended, for example during the night, and therefore it can also automatically match the barcodes/compound lists your screen and add results and analyzed them automatically in projects.

Don’t hesitate to contact us for more information and both virtual and real-life demonstrations where you get to run the instrument yourself after just a few hours of introduction – that is namely all it takes to safely and error-free handling of an instrument from Sophion.

Adaptive protocols at work

Charles River has presented how Qube with adaptive protocols gets tighter data on their Nav1.x panel when testing state-dependent sodium channel blockers. The performance was as good as with standard protocols but with 384 individual protocols, the modulatory effects of local anaesthetics were detected more reliably. Please click here to see the poster.

Cav1.2 on Qube, no rundown

High success rates and minimal rundown with pharmacology according to literature values. The third CaV1.2 cell line, this time kindly provided by Charles River, helps demonstrate how Ca-currents can be recorded stably on automated patch clamp. So, whether you want to investigate the CiPA protocol, interrogate for state dependent mode of action or biophysical characteristics, Qube is the enabling technology. See the full report here.

Qube is designed for drug discovery

We asked one of our customers how they screen several hundred thousands of compounds with high fidelity electrophysiology?

“We let the Qube run our large screening campaigns unattended during the night so we can run and develop other assays on the same instruments during the day”

Juha Kammonen, Principal Scientist, Charles River Laboratories

High throughput screening on Nav1.1 using Qube

The voltage-gated sodium channel NaV1.1 is highly expressed in fast spiking interneurons (FSIs), which are important for memory encoding and other cognitive functions. An impaired function of FSIs is associated with disorders like autism, schizophrenia, Alzheimer’s and others. Potentiators of NaV1.1 have been shown to minimize cognitive dysfunction in transgenic mice with decreased levels of NaV1.1 in parvalbumin-positive neurons and this target thus lend itself to therapeutic intervention. The challenge is to identify the molecule with the desired mode-of-action which inherently requires electrophysiology. In summary we show that Qube 384 provides:

  • Success rates up to 97%
  • Stable, unattended measurements over 7 hours
  • Consistent current voltage relationship
  • High reliability of detecting NaV1.1 activators

For the full application report please see here.