Step-by-step protocols for sophisticated, yet easy, screening on Qube
This article describes the basic procedure for setting up the screening protocol and recording data for Nav1.7 on a Qube automated patch‐clamp system. Three protocols along with step‐by‐step details are provided. First, we describe a protocol to estimate V½, the voltage at which half of the channels are inactivated, using traditional steady‐state inactivation measurement as well as a new adaptive online estimation. Second, we establish a state‐dependent protocol using adaptive online V½ measurement to obtain a concentration-response curve (CRC) on known reference blockers. Last, we introduce a use‐dependent protocol. In our hands, the sample reference demonstrated good state‐ and use‐dependent inhibition of Nav1.7.
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