Reduced cell consumption on QPatch ll and Qube 384 while maintaining high success rates - Sophion

Reduced cell consumption on QPatch ll and Qube 384 while maintaining high success rates


Author(s): Naja Møller Sørensen


Recommended cell concentration on both QPatch ll and Qube depends on both assay complexity, assay longevity and especially on cell quality. We generally recommend having a cell density of 3×106 cells/ml on both Qube and QPatch ll. However, the cell consumption for both systems can be reduced significantly, with a factor of at least 6, and still ensure success rates above 90%. We recommend that optimal cell concentrations are evaluated when working with cells that are expensive or in short supply. Furthermore, QPatch ll can now be loaded with as little as 50 μl cells for a single experiment.