hCav1.2 recordings using QPatch - Sophion

hCav1.2 recordings using QPatch

Author(s): Kadla R Rosholm, Melanie Schupp

The development of screening assays for the CaV1.2 channel has been challenging due to the tendency of the channel to exhibit declining current levels (rundown) during the experiment. Here we report a robust CaV1.2 assay yielding high success rates, low rundown and reliable pharmacology.

Pharmacology and current-voltage relationship in accordance with literature values

Success rates of up to 98%

Stable currents with rundown as low as 2% per min