How to differentiate induced pluripotent stem cells into sensory neurons for disease modelling: a functional assessment


Stem Cell Research & Therapy


Anil Kumar Kalia, Corinna Rösseler, Rafael Granja‑Vazquez, Ayesha Ahmad, Joseph J. Pancrazio, Anika Neureiter, Mei Zhang, Daniel Sauter, Irina Vetter, Asa Andersson, Gregory Dussor, Theodore J. Price, Benedict J. Kolber, Vincent Truong, Patrick Walsh and Angelika Lampert



Human induced pluripotent stem cell (iPSC)-derived peripheral sensory neurons present a valuable tool to model human diseases and are a source for applications in drug discovery and regenerative medicine. Clinically, peripheral sensory neuropathies can result in maladies ranging from a complete loss of pain to severe painful neuropathic disorders. Sensory neurons are located in the dorsal root ganglion and are comprised of functionally diverse neuronal types. Low efficiency, reproducibility concerns, variations arising due to genetic factors and time needed to generate functionally mature neuronal populations from iPSCs remain key challenges to study human nociception in vitro. Here, we report a detailed functional characterization of iPSC-derived sensory neurons with an accelerated differentiation protocol (“Anatomic” protocol) compared to the most commonly used small molecule approach (“Chambers” protocol). Anatomic’s commercially available RealDRG™ were further characterized for both functional and expression phenotyping of key nociceptor markers.

Keywords: Q2 2024

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