Pharmacological evaluation of GABAA receptor subtypes on Qube 384

Ligand-gated experiments require many compound additions for incubation, stimulation, test drug and combinations thereof. Enabling that in 384-format tremendously enhances the throughput which is necessary to find the interesting new NAMs, PAMs or other types of compounds.

Here we report studies of three subtypes of GABAA channels using Qube 384 and with focus on:

  • Short ligand exposure with repetitive stimulations with EC50 concentrations of GABA
  • Effects of agonists, antagonists and modulators
  • Cumulative and non-cumulative concentration-response relationships
  • Characterizing the pharmacological properties of four cell lines expressing different GABAA subtypes

For the full application report, please see here.