Academia and Automated Patch Clamp - Sophion

APC & Academia

Embracing Automated Patch Clamp

QPatch II – As the only platform on the market that can obtain gigaOhm seals in physiological solutions, combined with individual pressure pulse control, adaptive voltage protocols, glass-coated liquid channels, automated Rs compensation, and much more. QPatch II is the closest you get to manual patch clamp – just automated.

An increasing number of academic laboratories embrace automated patch clamp as a part of their laboratory tool pack. With increasing focus on deliverables and faster turn-around of research results automated patch clamp is a valuable addition to traditional manual patch clamp.

 

  • Hundred to a thousand-fold increase in data throughput
  • Standardized, easy-to-use and independent of user skills
  • Technical knowledge that goes beyond academia
  • Applicable on cell lines, stem cells, primary cells

“Although the MPC is still held as the benchmark for electrophysiologists, a major drawback is that it requires significant training and technical capability. This is becoming increasingly problematic in academia with a focus on deliverables and feasibility of research programmes”

(Bell and Dallas 2018)

Bringing your lab into the future

We want you to spend your time performing great science, not operating an instrument.

We believe that even advanced measurement solutions should be easy to use and our solutions are designed for many users and with amazing durability. Most of our first systems are 10-15 years old and still going strong. You can learn to operate the system in 10 minutes, so you can spend your time analyzing results or planning the next breakthrough experiments.

 

But can we afford it? While the systems can easily do the work of 10-20 skilled electrophysiology, the price does not follow suit. Let’s talk. You might be surprised

 

  • Spend your time on science, not on operating a rig or an instrument
  • Speed up your research with more data points, more replicates
  • Equip your PhDs and Post Docs with tools for a potential future in pharma
  • Built to last and built to learn

 

Qube 384 – With 384 individual amplifiers, stacker, Rs compensation, adaptive voltage protocols, temperature control and much more, Qube 384 is built for screening of large libraries, but also comes in handy when you have many clones, have a high cell-to-cell variation or just need many replicates for more challenging cells.

Core Laboratories – sharing is caring

 

While investment in APC equipment in the past has been reserved for pharma companies and a limited number of academic groups, sharing of equipment in core laboratories is getting more and more common.

  • Share technology across departments
  • Share knowledge across departments
  • Divide cost between departments

“One usage model that has been adopted by academia is that of a shared, core facility where several research groups club together to fund and generate the volume of experimental testing required“ 

(Bell and Dallas 2018)

Scientific value – no doubt about that

“A picture says more than a thousand words”. Same goes for a comprehensive list of peer-reviewed publications.

See below list of publications from academic institutions using QPatch and Qube 384.

 

Click here to learn more about our APC solutions – QPatch II and Qube 384

 

 

Publication list (from Academic institutions) 

– for a comprehensive list visit our publication database

 

Agwa, Akello J et al. 2018. “Efficient Enzymatic Ligation of Inhibitor Cystine Knot Spider Venom Peptides: Using Sortase A To Form Double-Knottins That Probe Voltage-Gated Sodium Channel Na(V)1.7.” Bioconjugate chemistry 29(10): 3309–19.

Agwa, Akello J. et al. 2020. “Manipulation of a Spider Peptide Toxin Alters Its Affinity for Lipid Bilayers and Potency and Selectivity for Voltage-Gated Sodium Channel Subtype 1.7.” Journal of Biological Chemistry 295(15): 5067–80. https://www.jbc.org/content/early/2020/03/05/jbc.RA119.012281.full.pdf.

Ai, Yong et al. 2015. “Synthesis and Biological Evaluation of Novel Olean-28,13β-Lactams as Potential Antiprostate Cancer Agents.” Journal of Medicinal Chemistry 58(11): 4506–20. https://doi.org/10.1021/jm5020023.

Al-Sabi, Ahmed et al. 2010. “Arrangement of Kv1 α Subunits Dictates Sensitivity to Tetraethylammonium.” Journal of General Physiology 136(3): 273–82.

Al-Sabi, Ahmed et al. 2017. “A Rational Design of a Selective Inhibitor for Kv1.1 Channels Prevalent in Demyelinated Nerves That Improves Their Impaired Axonal Conduction.” Journal of Medicinal Chemistry 60(6): 2245–56. https://doi.org/10.1021/acs.jmedchem.6b01262.

Arias, Hugo R et al. 2016. “Positive Allosteric Modulators of Α7 Nicotinic Acetylcholine Receptors Affect Neither the Function of Other Ligand- and Voltage-Gated Ion Channels and Acetylcholinesterase, nor β-Amyloid Content.” The International Journal of Biochemistry & Cell Biology 76: 19–30. http://www.sciencedirect.com/science/article/pii/S1357272516300930.

Bagchi, Bandita et al. 2014. “Disruption of Myelin Leads to Ectopic Expression of KV1.1 Channels with Abnormal Conductivity of Optic Nerve Axons in a Cuprizone-Induced Model of Demyelination.” PLoS ONE 9(2).

Bell, Damian C., and Mark L. Dallas. 2018. “Using Automated Patch Clamp Electrophysiology Platforms in Pain-Related Ion Channel Research: Insights from Industry and Academia.” British Journal of Pharmacology 175(12): 2312–21.

Billakota, Santoshi et al. 2019. “Personalized Medicine: Vinpocetine to Reverse Effects of GABRB3 Mutation.” Epilepsia 60(12): 2459–65.

Billet, Arnaud, Lionel Froux, John W. Hanrahan, and Frederic Becq. 2017. “Development of Automated Patch Clamp Technique to Investigate CFTR Chloride Channel Function.” Frontiers in Pharmacology 8(APR): 1–10.

Brown, Maile R. et al. 2016. “Physiological Modulators of Kv3.1 Channels Adjust Firing Patterns of Auditory Brain Stem Neurons.” Journal of Neurophysiology 116(1): 106–21.

Cardoso, Fernanda C. et al. 2015. “Identification and Characterization of ProTx-III [μ-TRTX-Tp1a], a New Voltage-Gated Sodium Channel Inhibitor from Venom of the Tarantula Thrixopelma Pruriens.” Molecular Pharmacology 88(2): 291–303.

Choi, Ryan et al. 2020. “Bumped Kinase Inhibitors as Therapy for Apicomplexan Parasitic Diseases: Lessons Learned.” International Journal for Parasitology. http://www.sciencedirect.com/science/article/pii/S0020751920300564.

Chow, Chun Yuen et al. 2020. “A Selective NaV1.1 Activator with Potential for Treatment of Dravet Syndrome Epilepsy.” Biochemical Pharmacology (February): 113991. https://doi.org/10.1016/j.bcp.2020.113991.

Chow, Chun Yuen et al. 2020. “Venom Peptides with Dual Modulatory Activity on the Voltage-Gated Sodium Channel NaV1.1 Provide Novel Leads for Development of Antiepileptic Drugs.” ACS Pharmacology & Translational Science 3(1): 119–34. https://doi.org/10.1021/acsptsci.9b00079.

Coleman, Nichole et al. 2014. “New Positive Ca2+-Activated K+ Channel Gating Modulators with Selectivity for KCa3.1.” Molecular Pharmacology 86(3): 342–57.

Daly, D et al. 2015. “Porphyrin Derivatives as Potent and Selective Blockers of Neuronal Kv1 Channels.” Chem. Commun. 51(6): 1066–69. http://dx.doi.org/10.1039/C4CC05639F.

Damann, Nils et al. 2020. “In Vitro Characterization of the Thermoneutral Transient Receptor Potential Vanilloid-1 (TRPV1) Inhibitor GRTE16523.” European Journal of Pharmacology 871: 172934. http://www.sciencedirect.com/science/article/pii/S0014299920300261.

Danahay, Henry L. et al. 2020. “TMEM16A Potentiation: A Novel Therapeutic Approach for the Treatment of Cystic Fibrosis.” American journal of respiratory and critical care medicine 201(8): 946–54. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7159426/.

Das, Pragnya et al. 2019. “Novel Chitohexaose Analog Protects Young and Aged Mice from CLP Induced Polymicrobial Sepsis.” Nature Scientific Reports 9(1): 1–12.

del Álamo, Juan C. et al. 2016. “High Throughput Physiological Screening of IPSC-Derived Cardiomyocytes for Drug Development.” Biochimica et Biophysica Acta – Molecular Cell Research 1863(7): 1717–27. http://dx.doi.org/10.1016/j.bbamcr.2016.03.003.

Deuis, Jennifer R. et al. 2016. “Analgesic Effects of GpTx-1, PF-04856264 and CNV1014802 in a Mouse Model of NaV1.7-Mediated Pain.” Toxins 8(3): 1–19.

Deuis, Jennifer R. et al. 2017. “Pharmacological Characterisation of the Highly Na v 1.7 Selective Spider Venom Peptide Pn3a.” Nature Scientific Reports 7(January): 1–19.

Diness, Jonas Goldin et al. 2017. “Termination of Vernakalant-Resistant Atrial Fibrillation by Inhibition of Small-Conductance Ca2+-Activated K+ Channels in Pigs.” Circulation: Arrhythmia and Electrophysiology 10(10): 1–13.

Flinspach, M. et al. 2017. “Insensitivity to Pain Induced by a Potent Selective Closed-State Nav1.7 Inhibitor.” Nature Scientific Reports 7(January): 1–16.

Franz, Denise, Hervør Lykke Olsen, Oliver Klink, and Jan Gimsa. 2017. “Automated and Manual Patch Clamp Data of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons.” Nature Scientific Data 4: 1–11.

Ghovanloo, Mohammad Reza et al. 2019. “Inhibitory Effects of Cannabidiol on Voltage-Dependent Sodium Currents.” Journal of Biological Chemistry 293(43): 16546–58.

Grillo, Alessandro et al. 2019. “Development of Novel Multipotent Compounds Modulating Endocannabinoid and Dopaminergic Systems.” European Journal of Medicinal Chemistry 183: 111674. https://doi.org/10.1016/j.ejmech.2019.111674.

Hammami, Sofia et al. 2009. “Cell Volume and Membrane Stretch Independently Control K+ Channel Activity.” Journal of Physiology 587(10): 2225–31.

Hao, Yuchen, Jingshu Tang, and KeWei Wang. 2015. “Development of Automated Patch Clamp Assay for Evaluation of Α7 Nicotinic Acetylcholine Receptor Agonists in Automated QPatch-16.” ASSAY and Drug Development Technologies 13(3): 174–84. https://doi.org/10.1089/adt.2014.622.

He, Linhong et al. 2018. “Design, Synthesis and Biological Evaluation of 7H-Pyrrolo[2,3-d]Pyrimidin-4-Amine Derivatives as Selective Btk Inhibitors with Improved Pharmacokinetic Properties for the Treatment of Rheumatoid Arthritis.” European Journal of Medicinal Chemistry 145: 96–112. http://www.sciencedirect.com/science/article/pii/S0223523417311108.

Hirsch, Rolf et al. 2020. “Antimicrobial Peptides from Rat-Tailed Maggots of the Drone Fly Eristalis Tenax Show Potent Activity against Multidrug-Resistant Gram-Negative Bacteria.” Microorganisms 8(5): 1–20.

Inserra, Marco C. et al. 2017. “Multiple Sodium Channel Isoforms Mediate the Pathological Effects of Pacific Ciguatoxin-1.” Nature Scientific Reports 7(January): 1–19.

Israel, Mathilde R et al. 2018. “The E15R Point Mutation in Scorpion Toxin Cn2 Uncouples Its Depressant and Excitatory Activities on Human NaV1.6.” Journal of Medicinal Chemistry 61(4): 1730–36. https://doi.org/10.1021/acs.jmedchem.7b01609.

Jalily, Pouria H. et al. 2020. “Put a Cork in It: Plugging the M2 Viral Ion Channel to Sink Influenza.” Antiviral Research 178(February): 104780. https://www.sciencedirect.com/science/article/pii/S0166354219305820.

Jenkins, David Paul et al. 2013. “Development of a QPatch Automated Electrophysiology Assay for Identifying KCa3.1 Inhibitors and Activators.” Assay and Drug Development Technologies 11(9–10): 551–60.

Jin, Ai Hua et al. 2015. “δ-Conotoxin SuVIA Suggests an Evolutionary Link between Ancestral Predator Defence and the Origin of Fish-Hunting Behaviour in Carnivorous Cone Snails.” Proceedings of the Royal Society B: Biological Sciences 282(1811).

Kaproń, Barbara et al. 2019. “Development of the 1,2,4-Triazole-Based Anticonvulsant Drug Candidates Acting on the Voltage-Gated Sodium Channels. Insights from in-Vivo, in-Vitro, and in-Silico Studies.” European Journal of Pharmaceutical Sciences 129: 42–57. http://www.sciencedirect.com/science/article/pii/S0928098718305530.

Klint, Julie K., Yanni K.Y. Chin, and Mehdi Mobli. 2015. “Rational Engineering Defines a Molecular Switch That Is Essential for Activity of Spider-Venom Peptides against the Analgesics Target NaV1.7.” Molecular Pharmacology 88(6): 1002–10.

Kramer, James et al. 2013. “MICE Models: Superior to the HERG Model in Predicting Torsade de Pointes.” Nature Scientific Reports 3: 1–7.

Kramer, James et al. 2020. “Cross-Site and Cross-Platform Variability of Automated Patch Clamp Assessments of Drug Effects on Human Cardiac Currents in Recombinant Cells.” Nature Scientific Reports 10(1): 1–15. http://dx.doi.org/10.1038/s41598-020-62344-w.

Kristof, Arnold S. et al. 2017. “An Official American Thoracic Society Workshop Report: Translational Research in Rare Respiratory Diseases.” Annals of the American Thoracic Society 14(8): 1239–47.

Łażewska, Dorota et al. 2017. “Biphenyloxy-Alkyl-Piperidine and Azepane Derivatives as Histamine H3 Receptor Ligands.” Bioorganic & Medicinal Chemistry 25(20): 5341–54. http://www.sciencedirect.com/science/article/pii/S0968089617310696.

Liao, Weike et al. 2020. “Design, Synthesis and Biological Activity of Novel 2,3,4,5-Tetra-Substituted Thiophene Derivatives as PI3Kα Inhibitors with Potent Antitumor Activity.” European Journal of Medicinal Chemistry 197: 112309. https://doi.org/10.1016/j.ejmech.2020.112309.

Loucif, Alexandre J.C. et al. 2018. “GI-530159, a Novel, Selective, Mechanosensitive Two-Pore-Domain Potassium (K 2P ) Channel Opener, Reduces Rat Dorsal Root Ganglion Neuron Excitability.” British Journal of Pharmacology 175(12): 2272–83.

Marcinkowska, Monika et al. 2016. “Design, Synthesis, and Biological Evaluation of Fluorinated Imidazo[1,2-a]Pyridine Derivatives with Potential Antipsychotic Activity.” European Journal of Medicinal Chemistry 124: 456–67. http://www.sciencedirect.com/science/article/pii/S0223523416307152.

Menegon, A. et al. 2017. “A New Electro-Optical Approach for Conductance Measurement: An Assay for the Study of Drugs Acting on Ligand-Gated Ion Channels.” Nature Scientific Reports 7(September 2016): 1–13.

Miner, Kent et al. 2019. “Drug Repurposing: The Anthelmintics Niclosamide and Nitazoxanide Are Potent TMEM16A Antagonists That Fully Bronchodilate Airways.” Frontiers in Pharmacology 10(FEB).

Mistry, Hitesh B., Mark R. Davies, and Giovanni Y. Di Veroli. 2015. “A New Classifier-Based Strategy for in-Silico Ion-Channel Cardiac Drug Safety Assessment.” Frontiers in Pharmacology 6(MAR): 1–6.

Mueller, Alexander et al. 2020. “Mapping the Molecular Surface of the Analgesic Na V 1.7-Selective Peptide Pn3a Reveals Residues Essential for Membrane and Channel Interactions.” ACS Pharmacology & Translational Science. https://pubs.acs.org/doi/abs/10.1021/acsptsci.0c00002.

Nausch, Bernhard et al. 2014. “NS19504: A Novel BK Channel Activator with Relaxing Effect on Bladder Smooth Muscle Spontaneous Phasic Contractions.” Journal of Pharmacology and Experimental Therapeutics 350(3): 520–30.

Okada, Jun Ichi et al. 2015. “Screening System for Drug-Induced Arrhythmogenic Risk Combining a Patch Clamp and Heart Simulator.” Science Advances 1(4): 1–8.

Okumu, Antony et al. 2020. “Novel Bacterial Topoisomerase Inhibitors Derived from Isomannide.” European Journal of Medicinal Chemistry 199: 112324. https://doi.org/10.1016/j.ejmech.2020.112324.

Ong, Seow Theng et al. 2019. “Extracellular K + Dampens T Cell Functions: Implications for Immune Suppression in the Tumor Microenvironment.” Bioelectricity 1(3): 169–79.

Petrou, Terry et al. 2017. “Intracellular Calcium Mobilization in Response to Ion Channel Regulators via a Calcium-Induced Calcium Release Mechanism.” Journal of Pharmacology and Experimental Therapeutics 360(2): 378–87.

Prashanth, Jutty Rajan, Nojod Hasaballah, and Irina Vetter. 2017. “Pharmacological Screening Technologies for Venom Peptide Discovery.” Neuropharmacology 127: 4–19. http://www.sciencedirect.com/science/article/pii/S0028390817301302.

Procopiou, Panayiotis A. et al. 2018. “Discovery of (S)-3-(3-(3,5-Dimethyl-1H-Pyrazol-1-Yl)Phenyl)-4-((R)-3-(2-(5,6,7,8-Tetrahydro-1,8-Naphthyridin-2-Yl)Ethyl)Pyrrolidin-1-Yl)Butanoic Acid, a Nonpeptidic Αvβ6 Integrin Inhibitor for the Inhaled Treatment of Idiopathic Pulmonary Fibrosis.” Journal of Medicinal Chemistry 61(18): 8417–43. https://doi.org/10.1021/acs.jmedchem.8b00959.

Rao, Vidhya R., Mathew Perez-Neut, Simon Kaja, and Saverio Gentile. 2015. “Voltage-Gated Ion Channels in Cancer Cell Proliferation.” Cancers 7(2): 849–75.

Reyes-Corral, Marta et al. 2019. “Differential Free Intracellular Calcium Release by Class II Antiarrhythmics in Cancer Cell Lines.” Journal of Pharmacology and Experimental Therapeutics 369(1): 152–62.

Robinson, Samuel D. et al. 2018. “A Comprehensive Portrait of the Venom of the Giant Red Bull Ant, Myrmecia Gulosa, Reveals a Hyperdiverse Hymenopteran Toxin Gene Family.” Science Advances 4(9): 1–13.

Shcherbatko, Anatoly et al. 2016. “Modulation of P2X3 and P2X2/3 Receptors by Monoclonal Antibodies.” Journal of Biological Chemistry 291(23): 12254–70.

Simó-Vicens, Rafel et al. 2017. “Effect of Antiarrhythmic Drugs on Small Conductance Calcium – Activated Potassium Channels.” European Journal of Pharmacology 803: 118–23. http://www.sciencedirect.com/science/article/pii/S0014299917302091.

Singh, Vinayak et al. 2017. “The Inosine Monophosphate Dehydrogenase, GuaB2, Is a Vulnerable New Bactericidal Drug Target for Tuberculosis.” ACS Infectious Diseases 3(1): 5–17.

Skarsfeldt, Mark A et al. 2016. “PH-Dependent Inhibition of K2P3.1 Prolongs Atrial Refractoriness in Whole Hearts.” Pflügers Archiv – European Journal of Physiology 468(4): 643–54. https://doi.org/10.1007/s00424-015-1779-0.

Solly, Kelli et al. 2015. “High-Throughput Screen of GluK1 Receptor Identifies Selective Inhibitors with a Variety of Kinetic Profiles Using Fluorescence and Electrophysiology Assays.” Journal of Biomolecular Screening 20(6): 708–19.

Sousa, Silmara R. et al. 2017. “Discovery and Mode of Action of a Novel Analgesic β-Toxin from the African Spider Ceratogyrus Darlingi.” PLoS ONE 12(9): 1–22.

Tay, Bryan et al. 2019. “Development of a High-Throughput Fluorescent No-Wash Sodium Influx Assay.” PLoS ONE 14(3): 1–14.

Tran, Hue N T et al. 2020. “Enzymatic Ligation of a Pore Blocker Toxin and a Gating Modifier Toxin: Creating Double-Knotted Peptides with Improved Sodium Channel NaV1.7 Inhibition.” Bioconjugate Chemistry 31(1): 64–73. https://doi.org/10.1021/acs.bioconjchem.9b00744.

Wacker, Sören J. et al. 2012. “Identification of Selective Inhibitors of the Potassium Channel Kv1.1-1.2(3) by High-Throughput Virtual Screening and Automated Patch Clamp.” ChemMedChem 7(10): 1775–83.

Walsh, Kenneth B. 2020. “Screening Technologies for Inward Rectifier Potassium Channels: Discovery of New Blockers and Activators.” SLAS Discovery.

Wang, Dan et al. 2018. “Synthesis of Pseudellone Analogs and Characterization as Novel T-Type Calcium Channel Blockers.” Marine Drugs 16(12): 1–12.

Wu, Chunhui et al. 2019. “Synthesis and Biological Evaluation of Five-Atom-Linker-Based Arylpiperazine Derivatives with an Atypical Antipsychotic Profile.” ChemMedChem 14(24): 2042–51. https://doi.org/10.1002/cmdc.201900439.

Xu, Mingshuo et al. 2018. “Synthesis and Biological Evaluation of a Series of Multi-Target N-Substituted Cyclic Imide Derivatives with Potential Antipsychotic Effect.” European Journal of Medicinal Chemistry 145: 74–85. https://doi.org/10.1016/j.ejmech.2017.12.099.

Yadav, Saurabh, Vishnu Kumar Dwivedi, Sarika Gupta, and Avadhesha Surolia. 2018. “Benzothiophenes as Potent Analgesics against Neuropathic Pain.” Advances in Experimental Medicine and Biology 1112: 245–54.

Yang, Daryl C. et al. 2016. “The Snake with the Scorpion’s Sting: Novel Three-Finger Toxin Sodium Channel Activators from the Venom of the Long-Glanded Blue Coral Snake (Calliophis Bivirgatus).” Toxins 8(10): 1–21.

Yang, Yang et al. 2016. “Scaffold Hopping toward Agomelatine: Novel 3, 4-Dihydroisoquinoline Compounds as Potential Antidepressant Agents.” Nature Scientific Reports 6(September): 1–20.

Zha, Chuantao et al. 2018. “Design, Synthesis and Biological Evaluation of Tetrahydronaphthyridine Derivatives as Bioavailable CDK4/6 Inhibitors for Cancer Therapy.” European Journal of Medicinal Chemistry 148: 140–53. http://www.sciencedirect.com/science/article/pii/S0223523418301442.

Zhang, Zhengping et al. 2016. “A Novel Acetylcholinesterase Inhibitor and Calcium Channel Blocker SCR-1693 Improves Aβ25–35-Impaired Mouse Cognitive Function.” Psychopharmacology 233(4): 599–613. https://doi.org/10.1007/s00213-015-4133-5.

Zhu, Fang et al. 2020. “Structural Optimization of Aminopyrimidine-Based CXCR4 Antagonists.” European Journal of Medicinal Chemistry 187: 111914. http://www.sciencedirect.com/science/article/pii/S0223523419310669.