hiPSC cardiomyocyte recordings using physiological solutions on QPatch ll

Induced pluripotent stem cell (iPSC) technology has created exciting new opportunities for cardiovascular research by providing a new platform to study the mechanisms of disease pathogenesis and evaluate cardiac drug safety.

It is challenging to get consistent APC recordings on hiPSC cardiomyocytes due to poor cell quality (high batch to batch variation), problematic getting high cell harvest purity, or inconsistent cell maturity.

This AR demonstrates that we can run hiPSC-CM experiments in physiological solutions with up to 50% success rates. Furthermore, we also demonstrate that it is possible to record paced action potentials with up to 20% success rates.

Read the entire report here.

The data was obtained as part of a collaboration with Professor Niels Voigt and his group at University Medical Center Göttingen, Germany.