Big steps in cardiomyocyte action potential recordings on QPatch II
In a fruitful collaboration between Prof. Niels Voigt and scientist Fitzwilliam Seibertz of The University of Göttingen and our research scientist Kadla Røskva Rosholm, PhD, we have made great strides in overcoming the maturation and recording hurdles in the research of human induced pluripotent and stem cell (hiPSCs).
With improved culturing and induction techniques, the Voigt lab have generated consistently ‘matured’ hiPSC-derived CMs. These maturation developments married with significant advances in QPatch II current clamp recordings have led to high quality recordings and success rates.
Action potential recordings were made in physiological Ringer’s solutions on our QPatch II instrument without using fluoride or calcium ‘seal enhancing’ solutions. Our developments on automated patch clamping provides techniques for fast, efficient data generation allowing accurate biophysical and pharmacological characterisation of hiPSC-CMs.
This collaborative data was recently presented at the Biophysical Society meeting. See the poster here