CRAC - Sophion
Activation of ICRAC by passive depletion in a thapsigargin assay on QPatch

CRAC

The calcium release-activated calcium (CRAC) channel plays a prominent role in many diseases. Molecules that modulate the activity of the CRAC current (ICRAC) can be useful for the treatment of a variety of disorders such as rheumatoid arthritis and multiple sclerosis, metastatic breast cancer, diabetes, inflammatory bowel disease, psoriasis, mast cell-related disorders, cardiovascular and cerebrovascular diseases and viral infections, as well as having potential to prevent transplant rejection.

 

CRAC channel assays have been seen as challenging on Automated Patch Clamp (APC) systems. A key challenge is the use of fluoride-containing ‘seal enhancers’. Fluoride is a no-go in assays investigating CRAC channels, and some manufacturers of APC equipment rely solely on seal enhancers to achieve good, high resistance (giga-Ohm) membrane seals, and good seals are critical to accurately and consistently measure very small ICRAC.

 

Way back in 2008, Sophion published the first application report showcasing a good assay for ICRAC performed on QPatch, without the need for fluoride seal enhancers. Since then, we have published more on this fascinating channel,  which is a potential target in many diseases. We also have a robust high throughput screening ICRAC assay  on Qube 384

Browse publications below or contact us for input and help.

Schematic overview of result from the Qube 384 ICRAC assay. Calcium current specified as the current which can be blocked by lanthanum

Posters

  • View Endogenous Ion Channels of Mammalian Cell Lines Characterization
    Year: 2008 First author: Friis et al., 2008
  • View Development of an Automated Patch Clamp Assay for recording STIM1/Orai1 – mediated currents using Qube 384
    Year: 2020 First author: Sauter et Al.

Papers

  • View Automated Patch-Clamp Technique: Increased Throughput in Functional Characterization and in Pharmacological Screening of Small-Conductance Ca2+ Release-Activated Ca2+ Channels
    Year: 2008 First author: Schrøder et al., 2008

Reports

  • View Icrac on QPatch
    Year: 2008 First author: Jensen et al., 2008

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