Stable response for ligand and voltage-gated ion channels on QPatch


Author(s): Olsen et al, 2010


The aim of this study is to observe the consistency of a current signal (elicited by an agonist or a voltage change) before and after antagonist or blocker application. This report presents data monitored on the automated QPatch 16 based on whole-cell current recordings from ligand-gated ion channels (LGIC); the glutamate-activated ligand-gated ion channels Glu5R expressed in HEK293 cells from Neurosearch and GluR6 (GRIK2) from Millipore as well as acid-sensing ion channel 3 (ASIC3) expressed in HEK293 cells. Similar experiments were performed on voltage-gated ion channels (VGIC); HEK293-Nav1.2a and CHO-hERG.