Fast series resistance compensation on Qube 384 and QPatch

Author(s): Jacobsen et al., 2016

Series resistance compensation for fast activating ion channels can help remove voltage clamp errors and increase clamp accuracy. All Sophions patch clamp amplifiers utilize a patented technique enabling up to 100% RS compensation. In addition, Sophion amplifiers are equipped with automatic clip detection to avoid loss of cells due to fatal oscillations, thereby increasing data throughput while maintaining high quality recordings. Here we show that voltage clamp errors can be up to 20-30 mV when uncompensated and in general that errors occur if not using RS compensation. However, we also show that the increase of accuracy due to use of RS compensation comes with a price of a lower success rate, and that the settings are critical to maximize success rates while avoiding or decreasing errors. Last we demonstrate the importance of automated clip detection.